FIGURE 6.

(A, B) Representative images of thioflavin‐S staining and quantification of the areas and fluorescence intensity and total numbers of Aβ plaques in the cortex and hippocampus; 5FAD, 5×FAD mice IV saline administration; 5FAD+DNT, 5×FAD+DNT, 5×FAD mice IV DNT administration. n = 15 slices from 3 mice per group; data are present as means ± SEM; ns, nonsignificant; *p < 0.05; **p < 0.01; ***p < 0.001; unpaired Student's t‐test. (C) Representative Western blots (left) and relative quantification (right) of Aβ‐42 expression levels in cortical tissues from the three groups. There was no significant reduction in Aβ‐42 deposition in 5×FAD and DNT‐treated mice. n = 3 mice in each group; mean ± SEM, ns, nonsignificant; *p < 0.05; one‐way ANOVA with Tukey's correction. (D) Based on the results (A and B), the quantities and frequency of plaques in the cortex and hippocampus of the two groups are shown in the histograms, every bar of the histogram represents the average values (upper) and average frequency [quantities of occurrence in the section/total number] of Aβ between the sections. Data are presented as means ± SEM; *p < 0.05; **p < 0.01; unpaired Student's t‐test. (E) Representative confocal images of Aβ1‐16 (6E10), lysosome (CD68), and microglia (IBA1) immunostaining in the cortex of the two groups of mice. n = 3 mice per group. Scale bar = 10 μm. Representative Western blots (right) and relative quantification of CD68 expression levels in cortical tissues from the three groups.