Table 1. Probe Concentration Optimization.
molar ratio (probe/tag) | linearity high end (ng) | standard and neg. control intersection (ng) | slope | R2 | SD% |
---|---|---|---|---|---|
3.00 | >350 | 9 | 63.6 | 0.977 | 4.92 |
1.50 | >350 | –1 | 50.7 | 0.9838 | 3.66 |
1.12 | 300 | –1 | 45.3 | 0.9793 | 3.13 |
0.75 | 300 | –20 | 35.1 | 0.9665 | 3.81 |