Figure 4.

MIF is required for LPS-induced Cox-2 expression, activity and suppression of apoptosis in murine peritoneal macrophages. (A) Resting peritoneal macrophages were stimulated for 18 h with 10 μg/ml LPS and 100 units/ml IFN-γ together with or without mouse rMIF. Results shown are the mean ± SD of duplicate samples (PGE₂) and are representative of two independent experiments. (B) Restoration of Cox-2 expression and activity by rMIF rescues MIF^−/− macrophages from augmented activation-induced apoptosis. Cells were treated as in A and assessed for apoptosis by ELISA of cytosolic oligonucleosomes as shown. Results shown are the mean ± SD of duplicate samples and are representative of three independent experiments. *, P < 0.04 for LPS/IFN-γ/MIF vs. LPS/IFN-γ treatment alone.