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. 2025 Jan 22;66(1):50. doi: 10.1167/iovs.66.1.50

Figure 4.

Figure 4.

DNA-PKcs inhibits the phosphorylation of ERK1/2. (A, B) iRPE cells were treated with erastin or 2 µM U0126 for four hours, the phosphorylation of ERK1/2 was determined by (A) Western blot and (B) quantitative analysis (n = 3). (C) Calcein/PI staining revealed PI+ iRPE cells treated with 20 µM erastin or 2 µM U0126 for 12 hours. Scale bar: 50 µm. (D) Quantitative analysis of the proportion of PI+ iRPE cells (n = 5). (E) Cell viability was analyzed by the CCK-8 after iRPE cells were treated with erastin or U0126 for 24 hours (n = 6). (F–H) The time courses of phosphorylation of DNA-PKcs and ERK1/2 were determined by (F) Western blot and (G, H) quantitative analysis after treated with 20 µM erastin (n = 3). (I, J) The iRPE cells were treated with 20 µM erastin or 1 µM KU-57788 for four hours, and the phosphorylation of ERK1/2 was determined by (I) Western blot and (J) quantitative analysis (n = 3). Data are mean ± SD, **P < 0.01, ***P < 0.001 using one-way ANOVA and post hoc Bonferroni's test.