Figure 7.

Appearance of GAD expression in locus coeruleus area of the groups of rats (A) Decreased expression in the control group, (B) Significantly increased expression in the alprazolam group (arrows), (C) Increased expression in L1 neurons compared to control group (arrow), (D) Significantly increased expression in L2 group (arrows), (E) Increased expression in L3 group neurons compared to control, Streptavidin biotin peroxidase method, Bars=50 µm. Appearance of GAD expression in preoptic area according to groups (A) decreased expression in neurons in the control group, (B) Significantly increased expression in neurons in the alprazolam group (arrows), (C) Increased expression in neurons of L1 group compared to control group (arrows), (D) markedly increased expression in L2 group (arrows), (E) increased expression in L3 group compared to control, Streptavidin biotin peroxidase method, Bars=50 µm. Appearance of GAD expression in the basal forebrain area of the groups (A) Decreased expression in the control group, (B) Significantly increased expression in the alprazolam group (arrows), (C) Increased expression in L1 neurons compared to control group (arrow), (D) markedly increased expression in L2 group (arrows), (E) increased expression in L3 group compared to control, Streptavidin biotin peroxidase method, Bars=50 µm