Table 2. Characterization of bacterial species in the external auditory canal: MALDI-TOF mass spectrometry for Gram-negative and Gram-positive species, and PCR for Mycoplasma species in cattle from PiraĆ (white background) and Castanhal (gray background) farms.
| Animal | Condition | Gram-positive | Gram-negative | Mycoplasma * |
|---|---|---|---|---|
| 1 | Infected | - | Escherichia coli | Mycoplasma bovis, Mycoplasma bovirhinis |
| 2 | Infected | - | Citrobacter koseri, E. coli | - |
| 3 | Infected | Staphylococcus cohnii | C. koseri, Serratia marcescens, E. coli | Mycoplasma spp. |
| 4 | Infected | - | - | Mycoplasma bovirhinis |
| 5 | Infected | Bacillus pumilus | S. marcescens, Acinetobacter lwoffi | Mycoplasma bovirhinis |
| 6 | Non-infected | Staphylococcus chromogens, Aerococcus viridans, Arthrobacter arilaitensis | - | - |
| 7 | Infected | Arthrobacter arilaitensis, S. chromogenes | - | - |
| 8 | Infected | - | - | Mycoplasma bovirhinis |
| 9 | Infected | - | - | - |
| 10 | Infected | Bacillus cereus | C. koseri, Oligella urethralis | - |
| 11 | Infected | - | - | - |
| 12 | Infected | S. chromogenes | C. koseri | Mycoplasma spp. |
| 13 | Infected | - | Morganella morgani | Mycoplasma bovis |
| 14 | Infected | - | M. morgani, S. marcescens | Mycoplasma bovis |
| 15 | Infected | - | S. marcescens | Mycoplasma spp. |
| 16 | Non-infected | - | - | - |
| 17 | Non-infected | - | - | - |
| 18 | Non-infected | Staphylococcus | - | - |
| saprophyticus, Arthrobacter gandavensis | ||||
| 19 | Non-infected | - | - | - |
| 20 | Infected | - | M. morgani | - |
Species detection was performed using PCR of the 5' UTR region.