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. 2001 Dec 18;99(1):407–412. doi: 10.1073/pnas.012587699

Figure 3.

Figure 3

Analyses of the plasma lipoproteins in hABCA1-Tg × apoE-KO and apoE-KO mice maintained on a regular chow diet. (A) The distribution of cholesterol in different FPLC elution fractions was analyzed after separation of the plasma lipoproteins present in pooled plasma from female apoE-KO (■; n = 5) and hABCA1-Tg × apoE-KO (○; n = 5) mice by FPLC. ApoA-I, apoA-II, and apoB present in FPLC fractions corresponding to VLDL (lanes 1 and 2), IDL/LDL (lanes 3), and HDL (lanes 4) elution volumes (16, 17, 22.5, and 31 ml, respectively) were determined by immunoblot analysis (Inset). (B) Two microliters of plasma from C57BL/6 (C), apoE-KO (E-KO), and hABCA1-Tg × apoE-KO (E-KO × hABCA1-Tg) mice were analyzed by native agarose gel electrophoresis followed by staining with fat red B. (C) Detection of apoA-I by immunoblotting after two-dimensional gel electrophoresis of plasma from apoE-KO and hABCA1-Tg × apoE-KO mice.