Fig. 1.

SsDps expression in response to oxidative stress. (A) Northern blot analysis of RNA extracted from exponential growth phase (OD₆₅₀ ≈0.5) S. solfataricus cells. Lane 1, standard growth conditions (0 μM H₂O₂₎; lane 2, 5 μM H₂O₂; lane 3, 10 μM H₂O₂; lane 4, 20 μM H₂O₂; lane 5, 30 μM H₂O₂. Approximately 1.5 μg of total RNA was loaded in each lane. (B) Western blot analysis of total protein extract from logarithmic (OD₆₅₀ ≈0.5) S. solfataricus cells. Loads are normalized according to number of cells, and lane assignments are as above. Densitometry and loading controls are available in Fig. 8.