Figure 2.
Spatial distribution of eIF-4E, 4E-BP1, 4E-BP2, and mTOR in hippocampal slices. Shown are confocal images of hippocampal slices stained with primary antibodies against purified rabbit IgG (control staining), eIF4-E, 4E-BP1, 4E-BP2, or mTOR proteins, and FITC-conjugated secondary antibodies. Note the staining signals for eIF-4E, 4E-BP1, 4E-BP2, and mTOR in cell bodies and dendrites. Because mTOR is mainly a membrane protein, little signal for this protein is detected in the cytoplasm of the cell body (asterisk). c, cell body; d, dendrites; s, stratum radiatum; m, molecular layer.