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. 2005 May 31;6:10. doi: 10.1186/1471-2091-6-10

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Copyright © 2005 Schrödel and de Marco; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Separation of recombinant GFP-GST fractions by a sucrose step gradient. A) Distribution of the recombinant protein using cell fractions recovered from different bacterial strains and from bacteria grown at different temperatures. Tube number 1 was loaded with the supernatant separated after lysate ultracentrifugation while total lysates were used for the other experiments. B) Dot-blot for the fractions separated by sucrose step gradient. Each fraction was tested with specific antibodies for the chaperones DnaK, ClpB, IbpB and GroEL.