REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Annexin V APC; 1:20	BioLegend	640920
7-AAD; 1:20	BioLegend	420404
Annexin V binding buffer	BioLegend	422201
CD3 monoclonal antibody (clone: SK7); 50 μg/mL	Invitrogen	16-0036-81
CD3 monoclonal antibody (clone: OKT3); 50 μg/mL	Invitrogen	16-0037-81
CD32 APC; 1:100	BioLegend	303208
Biological samples
Leukoreduction collars	Crimson Core (BWH)	N/A
Chemicals, peptides, and recombinant proteins
Concanamycin A	R&D Systems	2656
CellTrace CFSE	Thermo Fisher Scientific	65-0850-84
Dynabeads human T-activator CD3/CD28	Thermo Fisher Scientific	11131D
Recombinant human IFNβ	R&D Systems	8499-IF-010/CF
Recombinant human IL-15	R&D Systems	BT-015
Fetal bovine serum	GeminiBio	100-106
Gibco Penicillin-Streptomycin (10,000 U/mL)	Thermo Fisher Scientific	15140122
Gibco Gentamicin reagent solution	Thermo Fisher Scientific	15710064
Gibco L-glutamine	Thermo Fisher Scientific	25030081
Gibco Trypsin-EDTA 0.5% (10X)	Thermo Fisher Scientific	15400054
Gibco DMEM	Thermo Fisher Scientific	11966025
Gibco RPMI 1640 medium	Thermo Fisher Scientific	11875093
Gibco PBS (10X), pH 7.4	Thermo Fisher Scientific	70011069
Critical commercial assays
CD8+ Memory T Cell Isolation Kit, human	Miltenyi Biotec	130-094-412
CD8+ T Cell Isolation Kit, human	Miltenyi Biotec	130-096-495
Experimental models: Cell lines
L cells expressing CD32	Dr. Megan Levings, PhD	De Waal Malefyt et al.,8 Boardman et al.9
Software and algorithms
FlowJo	BD Biosciences	V10.10.0
Prism	GraphPad	V10.3.1
Other
BD Canto II	BD Biosciences	N/A
BD Fortessa	BD Biosciences	N/A
Nalgene Rapid-Flow Sterile single use vacuum filter units	Thermo Fisher Scientific	566-0020