Figure 4.
IFN-γ protein release ELISA following re-stimulated of 16-mer sensitized PBMCs from all donors with either the 9-mer or 10-mer determinant. Supernatants of 16-mer pp65340–355-sensitized PBMCs from CMV seropositive donors HLA-A*2402, A*0101, and Cw*0402 were collected at the three time points of 24 hours (black), 48 hours (orange), and 72 hours (yellow) after cell re-stimulation with either the 9-mer pp65341–349 or 10-mer pp65341–350 peptide. The figure shows the peptide induction and maintenance over time of the T lymphocyte reactivation from 16-mer pp65340–355-sensitized PBMCs after either 9-mer pp65 341–349 (9/16) or 10-mer pp65 341–350 (10/16) peptide recall compared to the specific 16-mer pp65340–355 re-induction (16) for each individual HLA association: HLA-A24 (top), HLA-A1 (middle), HLA-Cw4 (bottom).