Skip to main content
. 2001 Dec 18;99(1):483–488. doi: 10.1073/pnas.012593399

Figure 7.

Figure 7

Wt Pmp22 forms high molecular weight complexes with the mutant Pmp22s. COS7 cells expressing HA-tagged wt and Myc-tagged TrJ Pmp22 (A) or HA-tagged wt and Myc-tagged Tr Pmp22 (C) were lysed in Nonidet P-40 buffer and separated on an Amersham Pharmacia Superdex 200 column. An analysis of HA-tagged wt Pmp22 alone is shown in B. Fractions were analyzed by Western blot using antibodies against HA (rows A1, B, and C1) or Myc (rows A2 and C2). A significant amount of wt Pmp22 shifted from the monomer peak (B) into the dimer peak and high molecular aggregates in both mixtures (rows A1 and C1). The pattern of eluted TrJ Pmp22 remained unchanged (A2), whereas a significant amount of Tr Pmp22 shifted toward and into the dimer peak (C2). [Compare with the elution patterns of the homologous mutant Pmp22 proteins (Fig. 4, rows 2 and 3)].