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. 2024 Oct 8;12(1):uhae284. doi: 10.1093/hr/uhae284

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© The Author(s) 2025. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression analysis and characterization of MdNAC5^A and MdNAC5^T in apple. (A) Phenotype of ‘Orin’ calli overexpressing MdNAC5^A#1/2/3 and MdNAC5^T#1/2/3. (B) The protein levels of MdNAC5 transgenic apple calli. (C, D) Content of Met and ACC in MdNAC5 transgenic apple calli. (E) Relative expression of MdNAC5, MdERF3, and MdACS1 in the transcriptome of MdNAC5 transgenic apple calli. (F) Transient overexpression of MdNAC5 in ‘Fuji’ apple. Flesh firmness (G) and content of Met (H) and ACC (I) in MdNAC5 transient transformed apple fruit. (J) Relative expression of MdNAC5, MdERF3, and MdACS1 in MdNAC5 transgenic apple fruits. The bars are mean the values of three replicates (± SD), and diverse letters show differ significantly (Turkey’s test, P < 0.05).