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. 2001 Dec 26;99(1):495–500. doi: 10.1073/pnas.012589799

Figure 1.

Figure 1

Metabolic inhibition permeabilizes astrocytes to small positively and negatively charged fluorescent dyes; gap junction blockers prevent dye uptake. (Top) Phase contrast images. (Middle) EtdBr fluorescence. (Bottom) LY fluorescence. Before metabolic inhibition, confluent astrocyte cultures bathed with EtdBr (B) and LY for 2 min did not retain either dye (B and C, respectively). Astrocytes treated with 5 ng/ml antimycin A plus 0.3 mM iodoacetic acid for 75 min became permeable to both EtdBr and LY, as indicated by uptake of the dyes applied in the bath for 2 min (E, F). Sister cultures treated for the last 5 min of 75 min metabolic inhibition with either 1 mM octanol (G–I) or 35 μM AGA (J–L) did not take up either EtdBr (H and K) or LY (I and L).