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. 2025 Jan 4;11(1):e41625. doi: 10.1016/j.heliyon.2025.e41625

Table 1.

Gradient elution program for HPLC-MSn analysis.

Time (min) % Solvent A (0.1 % formic acid in water) % Solvent B (0.1 % formic acid in acetonitrile)
0 97 % 3 %
0–5 91 % 9 %
5–15 84 % 16 %
15–45 50 % 50 %