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. 2025 Jan 10;55(3):45. doi: 10.3892/ijmm.2025.5486

Figure 3.

Figure 3

Knockdown of klotho induces EMT in ARPE-19 cells. ARPE-19 cells were infected with lentiviral plasmids harboring sh-klotho or empty vector. (A) Klotho expression was measured using RT-qPCR following klotho overexpression. (B) Protein expression levels of klotho. (C) Quantification of the relative protein expression (normalized to GAPDH) in western blot. (D) Klotho expression was measured using RT-qPCR following knockdown. (E) Protein expression levels of EMT-related proteins were detected following klotho knockdown. (F) Quantification of the relative protein expression (normalized to GAPDH) in western blot. *P<0.05, **P<0.01, ***P<0.001 vs. sh-NC. sh, short hairpin; RT-q, reverse transcription-quantitative; NC, negative control; EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; ZO, zona occludens; Cad, cadherin; Lv, lentiviral vector.