Figure 1.

Arabidopsis atrbohD and atrbohF mutants. (A) Schematic representation of the transposon insertions in the Atrboh D and F genes. Boxes represent the exons of the genes, and red triangles mark the dSpm transposon insertions. (B) Representative 4-week-old rosettes. (C) Trypan blue staining of an atrbohD/F double mutant leaf, displaying spontaneous necrosis. (D) Detail of an atrbohD/F leaf stained with aniline blue to show callose deposition. Note that infection experiments are performed on young plants that do not exhibit ectopic cell death. [Bar = 1 cm (B) and 1 mm (D).]