Table 2. Summary of FlyLight adult fly line and image releases.

Includes descriptions of Jenett et al., 2012; Dionne et al., 2018; Tirian and Dickson, 2017; publications in Table 1; and stock distribution by Bloomington Drosophila Stock Center and Janelia Fly Facility. Section with italic text is specific to publications from Table 1 and this publication. Image counts are unique between categories, whereas line counts overlap extensively between categories. ‘Image tiles’ are considered as unique 3D regions, with each MCFO tile captured using two LSM image stacks. Stock shipments count each shipment of each stock separately. Raw and processed images are available at https://www.janelia.org/gal4-gen1, https://gen1mcfo.janelia.org, https://splitgal4.janelia.org, and many can be searched based on anatomy at https://neuronbridge.janelia.org.

	Gen1 GAL4	Gen1 LexA	Gen1 MCFO	AD/DBD	IS screen	SS screen	SS MCFO	SS polarity	Total
Lines	9389	1500	5155	8882	35,708	8986	7823	7050	67,562
Samples imaged	9389	1500	74,363	NA	36,182	16,974	55,702	37,526	231,636
20× image tiles	18,891	2966	29,780	NA	72,346	33,924	66,484	61,787	286,178
40× image tiles	0	0	111,563	NA	0	0	0	0	111,563
63× image tiles	0	0	22,372	NA	0	0	77,039	49,117	148,528
Samples with 63× images	0	0	8489	NA	0	0	31,849	17,619	57,957
Stocks currently available	2871	1781	NA	8358	NA	3013	NA	NA	16,023
Janelia & Bloomington stock shipments as of September 2024	167,082	29,227	NA	69,546	NA	40,067	NA	NA	305,922