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. 2024 Dec 24;14(1):2. doi: 10.3390/biology14010002

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Fluorescence observation of pollen tube growth of I. setosa after artificial self-pollination. (A) Pollen morphology under fluorescence, (B) Pollen germinated on the stigma at 1 h after pollination, (C) Pollen tube bundle growth at 2 h after pollination, (D) Pollen tube folds intertwined at 4 h after pollination, (E) Pollen tubes entered the style, (F) Pollen tube continues to grow downward, (G) Pollen tube bending, (H) Pollen tube fracture, (I) Pollen tubes reaches the ovule at 6 h after pollination. PG: Pollen grain, PT: Pollen tube, OU: Ovule.