Table 2: Plasmid DNA amounts for co-transfection in epegRNA architecture screen.

We typically use the TurboFectin 8.0 transfection reagent (OriGene #TF81005), but an alternative such as Lipofectamine3000 is suitable if it efficiently transfects HEK 293T cells.

Plasmid	DNA amount per well of 24-well plate
epegRNA-containing pU6-tevopreq1-GG-acceptor	75 ng
pCMV-PEmax-P2A-GFP	263 ng
pEF1a-hMLH1dn	132 ng