PCR Reaction Mastermix.
| Reagent | Amount |
|---|---|
| Oligo pool (20 ng/uL) | 3 |
| 2X iProof Mastermix | 12.5 uL |
| forward primer (10 uM) | 1.25 uL |
| reverse primer (10 uM) | 1.25 uL |
| ddH2O | 7 uL total volume |
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