Table 3.
qPCR primer pairs used to quantitate endogenous transcripts in this study
| Gene symbol | Forward/reverse qPCR oligo sequence | Optimal concentration | Amplicon | Intron spanned? | |
| Size | Tm | ||||
| Ank | 5'-AGTACCATAGTACACTCGGTTACCTGTCCTG-3' | 900 nM | 114 bp | 78.8°C | Yes |
| 5'-GCAAAGCTTTAAGTCGTAATCTAGCATCC-3' | 50 nM | ||||
| Axl/Ufo | 5'-CGACTACCTGCGTCAAGGAAATCG-3' | 300 nM | 112 bp | 82.8°C | Yes |
| 5'-AAAACTTGGCCGGTCTCGAGG-3' | 300 nM | ||||
| Cd34 | 5'-TGCTCTGGAATCCGAGAAGTGAGG-3' | 300 nM | 140 bp | 78.0°C | Yes |
| 5'-TCAGCCTCAGCCTCCTCCTTTTC-3' | 300 nM | ||||
| Dnchc1 | 5'-AACTAAACCCAGCCATTCGGCC-3' | 300 nM | 98 bp | 84.3°C | No |
| 5'-TTGCGTTGGCGGGTGACAG-3' | 900 nM | ||||
| Gap43 | 5'-GAGAAGGGAAGGAGAGAAGGCAGG-3' | 900 nM | 131 bp | 79.5°C | Yes |
| 5'-TCCGGCTTGACACCATCTTGTTC-3' | 900 nM | ||||
| Gapd | 5'-CGGAGTCAACGGATTTGGTCGTAT-3' | 900 nM | 214 bp | 82.6°C | Yes |
| 5'-GAAGATGGTGATGGGCTTCC-3' | 300 nM | ||||
| H19 | 5'-AGCTAACACTTCTCTGCTGCTCTCTGG-3' | 300 nM | 144 bp | 81.4°C | Yes |
| 5'-ATCTTCTTGATTCAGAACGAGACGGAC-3' | 900 nM | ||||
| Hand1 | 5'-GAGATGTATACCTGAGAGCAACAGGCATGATAGGTAG-3' | 300 nM | 113 bp | 75.1°C | No |
| 5'-CTTCTCCTTCATTTCTTTCCTTTTCCTTC-3' | 900 nM | ||||
| Hif1a | 5'-GTCAGCAGTACATGGTAGCCACAATTG-3' | 900 nM | 139 bp | 74.4°C | No |
| 5'-GATCCAGGCTTAACAATTCCATAGGC-3' | 300 nM | ||||
| Hmga1 | 5'-AATTCAGGAGGATGAACATCTGACGC-3' | 900 nM | 114 bp | 77.3°C | No |
| 5'-TCTGTTCACAAACTACCTCTGGACGG-3' | 50 nM | ||||
| Hprt1 | 5'-AACAATGCAAACTTTGCTTTCCCTG-3' | 300 nM | 123 bp | 80.1°C | Yes |
| 5'-TCAAATCCAACAAAGTCTGGCCTG-3' | 300 nM | ||||
| Igf2bp3 | 5'-AAGTATACATTCTCACAGAGACAGGATCGAGTGACTG-3' | 900 nM | 126 bp | 81.5°C | No |
| 5'-AAAGACAGATTTGCTTAACCAACAGACG-3' | 900 nM | ||||
| Lpl | 5'-TTTCCAGCCAGGATGCAACATTG-3' | 300 nM | 105 bp | 82.3°C | No |
| 5'-TGAATGGAGCGCTCATGCGAG-3' | 900 nM | ||||
| Myo1b | 5'-AATACACACCTTGTACCAATCAGCTCTCTC-3' | 900 nM | 143 bp | 76.1°C | No |
| 5'-TGATAAGAAGAGGCTGAGAGCCGTTC-3' | 900 nM | ||||