Table 3.
Genes binding Hsf in both Drosophila and S. cerevisiae
| FlyBase | Fly ratio | Yeast | Yeast express | NO-HS | HS | Lee ratio | Lee p | BLAST | Yeast GO function |
| Acon | 0.968 | ACO1 | 2.876 | 0.833 | 0.516 | 1.86 | 6.90E-02 | 4.00E-274 | Aconitate hydratase |
| Hsc70-5 | 1.686 | SSC1 | 1.98 | 0.999 | 0.999 | 7.05 | 1.50E-04 | 6.60E-201 | Protein transporter* |
| Hsp60 | 1.039 | HSP60 | 2.608 | 0.997 | 0.997 | 2.33 | 1.50E-02 | 1.00E-177 | Single-stranded DNA binding* |
| Cctgamma | 1.275 | CCT3 | -0.28 | 0.646 | 0.343 | 1.85 | 7.80E-02 | 4.00E-162 | Unfolded protein binding* |
| l(1)G0022 | 1.591 | CCT6 | 0.063 | 0.714 | 0.921 | 1.73 | 8.60E-02 | 1.00E-136 | Unfolded protein binding* |
| Hsc70Cb | 2.164 | SSE1 | 1.771 | 0.998 | 0.999 | 4.48 | 1.50E-03 | 1.70E-115 | Unfolded protein binding* |
| CG8863 | 0.818 | YDJ1 | 1.529 | 0.999 | 0.997 | 6.62 | 2.40E-04 | 2.00E-74 | Chaperone regulator* |
| Cyp1 | 1.805 | CPR1 | 1.251 | 0.996 | 0.995 | 10.83 | 3.40E-05 | 3.00E-65 | Peptidyl-prolyl cis-trans isomerase |
| CG8258 | 1.962 | CCT4 | NA | 0.385 | 0.055 | 1.85 | 7.80E-02 | 3.00E-55 | Unfolded protein binding* |
| CG2918 | 1.100 | SSA3 | 4.479 | 0.934 | 0.995 | 1.19 | 4.80E-01 | 7.00E-50 | ATPase |
| DnaJ-1 | 2.375 | SIS1 | 3.237 | 0.999 | 0.999 | 11.31 | 3.50E-05 | 7.00E-46 | Unfolded protein binding* |
| Rab35 | 1.416 | YPT32 | 0.338 | 0.269 | 0.343 | 1.98 | 4.30E-02 | 4.00E-40 | GTPase activity |
| sktl | 1.462 | MSS4 | 1.042 | 0.712 | 0.879 | 1.91 | 4.80E-02 | 7.00E-35 | Phosphatidylinositol kinase |
| CG4800 | 1.424 | RBF18 | 0.03 | 0.872 | 0.964 | 2.13 | 1.50E-01 | 1.00E-33 | Unknown |
| Cyt-c-d | 0.932 | CYC1 | 1.738 | 0.959 | 0.942 | 2.53 | 1.10E-02 | 4.70E-33 | Electron carrier |
| mbf1 | 0.706 | MBF1 | NA | 0.999 | 0.998 | 9.4 | 1.80E-04 | 2.00E-29 | Transcription coactivator |
| CG1416 | 2.793 | AHA1 | 3.288 | 0.999 | 0.999 | 9.14 | 5.60E-04 | 3.00E-28 | Chaperone activator* |
| CG4500 | 1.37 | FAA1 | 3.889 | 0.796 | 0.943 | 2.61 | 8.00E-03 | 9.00E-23 | Fatty-acid-CoA ligase |
| CG32920 | 1.081 | AHP1 | 1.547 | 0.983 | 0.984 | 3.81 | 1.10E-03 | 1.10E-19 | Thioredoxin peroxidase |
| SH3PX1 | 0.877 | SNX4 | 1.26 | 0.824 | 0.372 | 2.52 | 1.30E-02 | 6.30E-13 | lipid binding |
| CG10973 | 1.532 | FES1 | 3.541 | 0.997 | 0.993 | NA | NA | 1.10E-10 | Adenyl-nucleotide exchange factor |
| l(2)35Bg | 0.755 | DRE2 | 0.304 | 0.843 | 0.832 | 3.36 | 3.70E-03 | 5.00E-10 | Unknown |
| CG12200 | 0.786 | CST9 | 1.022 | 0.346 | 0.467 | 1.69 | 9.40E-02 | 8.70E-10 | DNA binding |
The FlyBase gene ID is given, along with the average asinh ratio of Hsf enrichment. Yeast homologs are indicated by their Saccharomyces genome database (SGD) common names. The average heat-induced expression and ranking of Hsf binding in non-heat shocked (NO-HS) and heat shocked (HS) cells are from [28], the Lee ratio and Lee p are from [27]. BLAST scores are derived from searches at the SGD using the Drosophila sequences as probes. Gene Ontology (GO) functional classifications are for the yeast proteins; asterisks indicate stress-response proteins.