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. 2005 Jul 15;19(14):1662–1667. doi: 10.1101/gad.1323805

Figure 3.

Figure 3.

PBAF and BAF complexes are selectively required for expression of distinct IFN-α-inducible genes. (A) RT–PCR shows that HeLa cells depleted of BAF200 by vector-based siRNA display drastically reduced expression of the IFITM1 gene. β-Actin was included as a control. (B) RT–PCR shows that HeLa cells depleted of BAF200 by siRNA oligos exhibited strong inhibition of IFITM1 gene expression, but a normal level of IFITM3 gene. (C) RT–PCR shows that HeLa cells depleted of BAF250a by siRNA oligos have significantly reduced expression of IFITM3 gene under both basal and IFN-α-induced conditions. (D) Immunoblotting shows that BAF250a was efficiently depleted by siRNA oligos in HeLa cells. A nonspecific siRNA oligo was included as a control. Immunoblotting of BAF57 was used as a loading control.