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. 2005 Jul 15;19(14):1662–1667. doi: 10.1101/gad.1323805

Figure 5.

Figure 5.

BAF200 forms a complex with BRG1 and mediates IFITM1 gene expression in the absence of BAF180. (A) Immunoblotting shows that BAF200 from the extract of BAF180-deficient HCC1143 cells cofractionates with BRG1 and BAF57. (B) Immunoblotting shows that BAF200 in the extract of HCC1143 cells coimmunoprecipitates with BRG1, and BAF57. The preimmune serum (Pre) was used as a control. (C) A diagram of the purification scheme for PBAF and BAF complexes. (D) A silver-stained SDS-gel shows PBAF and BAF complexes immunoisolated with BRG1 antibody from the extract of HCC1143 cells and HeLa cells. An “unknown” polypeptide in the PBAF complex of HCC1143 cells was labeled with an asterisk. (E) Immunoblotting shows that BAF200 expression is suppressed by siRNA oligos in HCC1143 cells. BAF57 was used as a loading control. (F) RT–PCR shows that HCC1143 cells depleted of BAF200 by siRNA oligos have reduced the expression of the IFITM1 gene under both basal and IFN-α-induced conditions.