Figure 2.

Mitotic asynchrony in early ago-2 embryos. Wild-type and ago-2 embryos were stained with DNA dye, Hoechst (imaged in green), and phospho-histone H3 (pH3) antibody (imaged in red). (A) Presyncytial blastoderm wild-type embryo showing synchronously dividing, evenly spaced nuclei that have pH3. A close-up from same embryo showing merged (B) and pH3 (C) staining. (D) Presyncytial blastoderm ago-2 embryo showing unevenly spaced and asynchronously dividing nuclei. A close-up from the same embryo shown in panels E and F. Arrows in panel E indicate asynchronous “micronuclei.” (G) Wild-type syncytial blastoderm embryo showing synchronously dividing nuclei. A close-up from the same embryo showing merged (H) and pH3 (I) staining. (J) ago-2 syncytial blastoderm embryo showing asynchronously dividing nuclei. (K,L) A close-up from the same embryo. Note that only a subset of nuclei is positive for pH3. Arrowhead in panel L indicates incompletely separated asynchronous nuclei.