Figure 1.

Phenotype analysis of mycobacterial strains overexpressing pknA or pknB. Growth and viability of M. smegmatis (A) and M. bovis BCG (B) containing a single additional copy of pknA (filled gray circle), pknB (▵) pknAK2M (•), pknBK40M (▴) or plasmid only (▪), under the control of the acetamide-inducible promoter. Cultures were grown to early log phase, diluted to OD₆₀₀ = 0.02 and cultured in the presence of inducer. Error bars are ±1 SD. In the bar graph, the pknB-overexpression strain is shown in white, the pknA-overexpression strain in gray, and the control in black. (C) Negative stain electron micrographs of M. smegmatis and M. bovis BCG cells containing a single additional copy of pknA, pknB, or plasmid under the control of the acetamide-inducible promoter in pMH94. Cultures were grown in the presence of inducer to early stationary phase, at which point they were harvested, fixed, and stained. Bar, 500 nm.