Figure 3.

The severe atrophy of denervated runx1 mutant myofibers is not accompanied by excessive activation of the FoxO or NFκB pathways. Following denervation, the extent of Fbxo32 (a.k.a. atrogin-1, MAFbx) and MuRF1 induction is similar in MCK::cre; runx1^f/f and control runx1^f/f mice. As Fbxo32 is a target of FoxO and MuRF1 is induced by NFκB, these results indicate that FoxO and NFκB are similarly activated in denervated muscles from MCK::cre; runx1^f/f and control runx1^f/f mice. The level of Fbxo32, MuRF1, and Gapd expression in innervated (Inn) and denervated (Den) (3-d) muscles was measured by an RNase protection assay (Zhu et al. 1994).