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. 2005 Jul 15;33(12):3866–3874. doi: 10.1093/nar/gki698

Figure 1.

Figure 1

UV crosslinking of CUG repeat RNAs in HeLa nuclear extracts. (A) RNA clones used. The fragments of the DMPK gene with 5, 46 or 85 CTG repeats were cloned and transcribed in vitro with T7 RNA polymerase. Black bars represent the vector sequence common to all three clones. The 3′ branch site is underlined. (CUG)85′ is the clone with 85 repeats of CUG and a mutated branch site. (B) UV crosslinking using HeLa nuclear extracts. Lane 1, (CUG)5; lane 2, (CUG)85; lane 3, (CUG)46; lane 4, biotinylated (CUG)46 (underlined); lane 5, with biotinylated (CUG)85 (underlined). (C) UV crosslinking in DM extracts. Lanes 1 and 2, HeLa total cell extracts; lanes 3 and 4, total DM1 cell extracts before cell differentiation; lanes 5 and 6, DM1 extracts after differentiation.