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. 2005 Jul 15;33(12):3866–3874. doi: 10.1093/nar/gki698

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Purification and identification of the CUG repeat binding protein. (A) Purification of the binding protein. The eluted proteins from the RNA affinity column using the (CUG)46 or (CUG)85 RNAs were separated in a SDS–PAGE gel. (B) UV crosslinking assays in extracts treated with pre-immune (pre) or hnRNP H anti-sera (post). (C) The crosslinking products were treated with pre-immune sera (pre, lane 2) or anti-hnRNP H (post, lane 3). (D) The levels of hnRNP H and beta-actin were compared from cells that were mock-transfected or transfected with anti-hnRNP H siRNAs (top panel). The siRNA-treated cell extracts were used in UV crosslinking assays (bottom panel).