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. 2005 Jul 15;33(12):3932–3941. doi: 10.1093/nar/gki712

Figure 6.

Figure 6

The C-terminal domain of BLM, between residues 1290 and 1350, is required for ssDNA annealing. (a) Strand annealing as a function of increasing protein concentration for BLM213–1417 and BLM213–1267. Reactions were incubated for 30 min. The graph below shows quantification of the data. (b) Time course of ssDNA annealing by 20 nM BLM213–1417 or BLM213–1267. The graph below shows quantification of the data. (c) Comparison of the helicase activity of BLM213–1417 and BLM213–1267. Assays were as described in Figure 1. Graph below shows quantification of the data. (d) Strand annealing as a function of increasing protein concentration for BLM642–1290 and BLM642–1350. Graph below shows quantification of the data.