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. 2002 Jun;13(6):1806–1818. doi: 10.1091/mbc.01-08-0399

Figure 1.

Figure 1

CPY* degradation is impaired in mutants defective in ER-to-Golgi transport. Pulse-chase analysis was performed to measure CPY* degradation and maturation of PrA in wild-type and isogenic mutant strains. Cells were shifted to restrictive temperature 5 min before the chase and lysed at the indicated time points. CPY* or PrA were immunoprecipitated and separated by SDS-PAGE. Quantification was done using a PhosphorImager. (A) Formation of COPII-coated vesicles (Sec23p) and ER and Golgi t-SNARES (Ufe1p and Sed 5p) are necessary for efficient degradation of CPY*. (B) CPY* degradation requires a functional Sar1p activating factor (Sec12p) and yeast NSF (Sec18p). (C and D) Transport is blocked in the mutant strains as evidenced by the maturation defect of PrA (pPrA, p1 PrA precursor of the ER; mPrA, mature PrA of the vacuole).