Figure 2.

(A) Immunolocalization of CHO1 in CHO cells at different stages of mitosis. After synchronization, cells were fixed at the different stages of mitotic progression and stained with DAPI (a–g), antitubulin (a′–g′) and polyclonal anti-CHO1 (a"–g") antibodies. CHO1 changes its distribution from the entire spindle (a") to the midzone region during anaphase (b"–f") and concentrates to a midbody at the end of cytokinesis (g"). (B) Distribution of exogenous CHO1F in transfected cells is identical to the distribution of endogenous CHO1. Cells fixed at metaphase (a, a′), anaphase (b, b′), and telophase (c, c′) were stained with anti-HA (a, b, and c) and polyclonal anti-CHO1 (a′, b′, and c′) antibodies. Bar, 10 μm.