Figure 4.

High level of CHO1F′ expression inhibits completion of cytokinesis in CHO cells. Although the midzone bundles are forming in cells expressing CHO1F′ (A′ and A"), the daughter cells remain connected by the cytoplasmic bridges after mitosis (B–F). Cells expressing mutant protein are stained by anti-HA (A′, B, C, D′, E′, and F) and either anti-CHO1 polyclonal (A", B′, D", and F′) or antitubulin (C′ and E") antibodies. DNA is visualized by DAPI staining (A and E). Bars, 10 μm