Figure 6.

Mutant CHO1 inhibits the accumulation of endogenous CHO1 at the midzone/midbody region. (A–D) The effect of CHO1F′ΔE20 expression on the localization of endogenous CHO1. When the expression of CHO1F′ΔE20 increases from low (A) to medium (B) and high levels (C), the endogenous CHO1 becomes depleted from midzone/midbody area of the dividing cells (arrows in B′ and C′). (D–D") Three anaphase cells expressing different levels of mutated protein in the same field. The higher the level of CHO1F′ΔE20 expression (D), the weaker the accumulation of endogenous protein at the midzone region (arrows 1 to 3 in D′). Staining was performed using anti-HA (A–D) and anti-CHO1 E20 (A′–D′) antibodies and DAPI (A"–D"). Bars, 10 μm.