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. 2002 Jun;13(6):1832–1845. doi: 10.1091/mbc.01-10-0504

Figure 7.

Figure 7

Formation of stem body and midbody matrices is inhibited in dividing cells expressing mutated CHO1. CHO cells, transfected with CHO1F (A and C) and CHO1F′ (B and D) constructs were fixed at anaphase (A and B) and telophase (C and D) stages and stained using anti-HA (A–D), antitubulin (A′–D′) antibodies, and DAPI (A"–D"). The stem body and midbody matrix is easily detected in cells expressing wild-type CHO1F (arrowheads in A′ and C′). However, in cells expressing mutant protein, the dense matrix material is missing, based on the absence of dark spots in tubulin staining (arrows in B′ and D′). Bars, 10 μm.