Table 1.

Expression of CHO1F′ causes multinucleation in CHO cells

Expressed DNA constructs	Cells with more than one nucleus (%)	p value	Number of counted cells
CHO1F′	44.6  ± 11.2	0.05	3  × 500
CHO1F	7.8  ± 6.7	0.05	3  × 500
No expression (control)	1.5  ± 0.6	0.01	6  × 500

Cells were transfected with CHO1F and CHO1F′ constructs and cultured for 48 h before fixation. Fixed cells were immunostained with anti-HA antibodies to visualize cells expressing exogenous proteins. The number of nuclei was counted in 500 cells expressing CHO1F and CHO1F′ constructs and in 500 nonexpressing (control) cells, present on the same coverslips. Experiment was repeated three times.