Figure 5.

Immunofluorescence staining of mouse skin and esophagus after ex vivo inhibition of protein phosphatases. Fragments of mouse ear skin and esophagus were treated for 2 h with 1 μM Cl-A (c, d, g, and h) or DMSO (a, b, e, and f; controls). Tissue sections were triple labeled for DL15/K6/nuclei (a and c), LJ4/K5/nuclei (b, d, f, and h), and DL15/K4/nuclei (e and g). Toto-3 iodide was used to stain nuclei, shown in blue (a–h). DL15 and LJ4 staining is shown in green, whereas K4–K6 staining is shown in red. Arrows point from suprabasal toward the basal epithelial layer. HF, hair follicle; L, lumen.