Figure 7.
K5 and K6 LLTPL motif is phosphorylated during cell stress. Confluent primary cultured keratinocytes were treated with UV-C (400 J/m2; 0.2, 1, and 24 h), An (10 μM; 1 and 24 h), or MMS (1 mg/ml; 24 h). Control cells (24 h) were cultured without any manipulations. Total cell lysates were separated on SDS-PAGE, transferred to membranes, and then blotted using DL15, LJ4, and antibodies to K5 and K6. Equal protein loading was confirmed by Coomassie blue staining (unpublished data). The predicted K8 migration position is indicated by gray marking.