Figure 6.

Model for centrosome reorientation in wound edge cells. (a) In CHO cells, most microtubules (blue lines) are focused at the centrosome (pink circle) and extend to the cell periphery. Activated motors in the cortex (red squares) contribute to centrosome repositioning; the microtubules and centrosome reorient as a unit. Photoactivated marks on the microtubules are shown in green. Blue arrow indicates location of wound. (b) In PtK cells, noncentrosomal microtubules persist due to the presence of stabilizing minus-end caps (not illustrated). Slower turnover results in fewer interactions between centrosomal microtubules and cortical motors. Microtubule are transported individually to the front of the cell.