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Stu1p is necessary for spindle maintenance. stu1–5 cells expressing Spc42-GFP (CUY1158) were arrested with hydroxyurea at 22°C (A and B) and shifted to 37°C for 30 min in the continued presence of hydroxyurea (C and D). (A and C) DIC. (B and D) Spc42-GFP.
