Figure 6.

Identification of the Stu1p microtubule-binding domain. Various truncated Stu1p polypeptides were translated in vitro and tested for microtubule-binding activity by the cosedimentation assay using a constant amount of microtubules (5 μm tubulin). The percentage of Stu1p that pelleted with microtubules is indicated by (+), >80%; (±), 60–80%; (∓), 40–60%; and (−), <20%. The K_ds of some of these Stu1p polypeptides were determined as shown for full-length Stu1p in Figure 4. The K_ds shown are the average values from two separate assays, and the range is indicated. The top line represents the full-length Stu1p. The numbers adjacent to the end points represent the positions of the corresponding amino acids.