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Subcellular localization of granuphilin-a and -b. INS-1 cells transiently transfected with myc-tagged granuphilin-a (A, C, E) or -b (B, D, F) were grown for 2 days on glass coverslips coated with laminin and poly-l-lysine. The cells were fixed and analyzed by confocal microscopy using an anti-myc antibody and an anti-insulin antibody. (A and B) Anti-myc; (C and D) anti-insulin; (E and F) overlay of the signal obtained with the anti-myc and anti-insulin antibodies.
