Table 5.
Accuracies and precisions of the developed method in diluted human plasma
| Name | Within-run accuracy (% bias) |
Within-run precision (% CV) |
Between-run accuracy (% bias) |
Between-run precision (% CV) |
||||
|---|---|---|---|---|---|---|---|---|
| Q1 | Q2 | Q1 | Q2 | Q1 | Q2 | Q1 | Q2 | |
| 7-keto-25-OHC | −10.5 | 6.1 | 3.5 | 2.9 | 17.0 | 15.1 | 2.7 | 6.1 |
| 7-keto-27-OHC | −26.9 | 0.0 | 5.0 | 4.7 | 17.2 | 28.6 | 3.6 | 11.1 |
| 7α,24 (S)-diOHC | −2.3 | −0.6 | 3.9 | 6.5 | −9.0 | −12.2 | 14.6 | 17.2 |
| 7α,25-diOHC | −9.4 | −6.8 | 2.2 | 6.0 | 12.1 | 10.4 | 10.0 | 9.6 |
| 7α,27-diOHC | −12.3 | −7.4 | 10.8 | 6.0 | 5.5 | 11.4 | 10.2 | 10.7 |
| 5α,6β-diOHC | −31.9 | −21.2 | 1.9 | 3.5 | 4.5 | 10.6 | 5.8 | 11.0 |
| 7α,12α-diOHCone | −26.9 | 0.0 | 5.0 | 4.7 | 17.2 | 28.6 | 3.6 | 11.1 |
| 7-ketochol | −5.7 | −16.2 | 10.1 | 3.9 | −10.9 | 9.2 | 22.6 | 13.6 |
| 4β-OHC | 13.4 | 2.3 | 4.9 | 9.2 | 6.6 | 5.6 | 11.6 | 13.0 |
| 7α-OHC | - | - | - | - | - | - | - | - |
| 22 (R)-OHC | −1.4 | −0.6 | 3.2 | 3.8 | 14.3 | 12.7 | 6.0 | 8.0 |
| 24 (S)-OHC | −14.1 | −18.4 | 1.3 | 4.5 | 7.4 | 9.7 | 7.3 | 8.6 |
| 25-OHC | −12.8 | −11.4 | 1.3 | 3.5 | 6.8 | 9.5 | 8.7 | 12.5 |
| 27-OHC | −8.4 | −10.7 | 1.8 | 2.7 | 3.7 | 8.7 | 7.5 | 10.5 |
| 7α-OHCone | −12.1 | −3.1 | 1.9 | 2.1 | 4.4 | 15.5 | 10.3 | 11.0 |
| 5α,6α-epoxychol | 6.6 | −9.5 | 8.9 | 10.6 | 15.4 | −6.9 | 23.1 | 24.7 |
| 5β,6β-epoxychol | −5.7 | −17.3 | 8.9 | 9.5 | 16.4 | 10.0 | 30.1 | 28.1 |
| 24 (S),25-epoxychol | −4.3 | 9.1 | 9.3 | 7.7 | 6.6 | 18.0 | 7.5 | 10.1 |
| α-MCA | −0.1 | −7.1 | 3.6 | 2.9 | −6.4 | −1.7 | 10.0 | 10.5 |
| β-MCA | 3.7 | −4.5 | 4.1 | 3.1 | −4.4 | −0.5 | 8.7 | 7.8 |
| ω-MCA | −2.2 | −7.5 | 3.4 | 2.7 | −7.4 | −2.9 | 6.6 | 7.0 |
| HCA | −9.4 | −11.1 | 3.6 | 2.9 | −9.9 | −0.8 | 11.7 | 13.0 |
| CA | −4.9 | −1.2 | 0.9 | 2.1 | −2.4 | −0.3 | 9.2 | 7.9 |
| CDCA | −13.3 | −11.8 | 2.6 | 0.9 | −5.3 | 2.1 | 10.1 | 9.4 |
| HDCA | −17.9 | −19.4 | 3.2 | 2.2 | −8.3 | 0.8 | 11.5 | 14.0 |
| MDCA | −17.0 | −18.8 | 3.3 | 4.0 | −13.4 | −5.0 | 8.6 | 11.6 |
| UDCA | −22.3 | −17.7 | 3.3 | 3.1 | −13.2 | −2.0 | 10.5 | 14.8 |
| DCA | −38.0 | −23.0 | 1.8 | 15.1 | −8.8 | −4.4 | 11.9 | 10.5 |
| LCA | −6.4 | −8.4 | 3.0 | 4.2 | −1.5 | 2.7 | 1.7 | 3.6 |
| Tα-MCA | −12.5 | −7.8 | 2.7 | 1.2 | −9.6 | −6.1 | 2.7 | 5.1 |
| Tβ-MCA | −13.4 | −13.0 | 3.0 | 2.5 | −10.4 | −2.9 | 3.3 | 9.6 |
| Tω-MCA | −14.2 | −10.8 | 4.7 | 1.8 | −7.7 | −5.3 | 4.9 | 5.8 |
| TCA | −7.0 | −6.0 | 1.0 | 1.8 | −2.3 | −0.5 | 2.1 | 4.0 |
| TCDCA | −23.6 | −18.6 | 0.5 | 0.9 | −5.6 | −3.6 | 1.9 | 8.3 |
| THDCA | −25.7 | −4.9 | 2.9 | 2.2 | −10.6 | −4.9 | 3.4 | 13.0 |
| TUDCA | −18.4 | −14.3 | 2.7 | 1.2 | −6.5 | −3.1 | 2.3 | 10.1 |
| TDCA | −25.2 | −21.4 | 1.9 | 1.6 | −6.8 | −2.9 | 1.9 | 9.3 |
| TLCA | −14.6 | −13.1 | 0.8 | 1.1 | 5.1 | −0.2 | 2.0 | 3.7 |
| GCA | −10.6 | −8.1 | 0.5 | 3.0 | −7.3 | −5.2 | 11.7 | 10.1 |
| GCDCA | −13.3 | −2.9 | 6.9 | 4.8 | −0.7 | 1.3 | 15.7 | 15.8 |
| GHDCA | −12.8 | −14.1 | 4.1 | 2.8 | 4.2 | 0.0 | 13.3 | 11.5 |
| GUDCA | −16.9 | −15.1 | 3.3 | 1.8 | −4.1 | −8.1 | 10.6 | 9.4 |
| GDCA | −17.4 | −17.5 | 1.2 | 5.9 | −0.6 | −2.2 | 9.0 | 8.7 |
| GLCA | 2.5 | −4.8 | 7.1 | 2.2 | 8.2 | 0.5 | 10.0 | 7.8 |
| THCA | 4.3 | 4.9 | 1.5 | 2.9 | −6.3 | −6.0 | 11.2 | 8.7 |
This table shows the within-run and between-run accuracies (expressed as % bias) and precisions (expressed as % CV) of the described method in diluted human plasma (20 μl in 180 μl PBS). Spiked amounts (Q1 and Q2) of pure standards are available in supplemental Table S1.