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. 2002 Jun;13(6):1940–1952. doi: 10.1091/mbc.02-02-0016

Figure 5.

Figure 5

Transcriptional activity of MyoD and MEF2 is activated by the CaMK- and calcineurin-mediated signaling pathways. (A and B) C2C12 cells were transfected with either 3MEF2-luc (A) or 4RE-luc (B) in combination with either an empty vector, the constitutively active CaMKIVc, or CnAΔ. (C) 10T1/2 cells stably expressing MyoD were transfected with 4RE-luc along with various plasmids as indicated. (D) HeLa cells were transfected with gal4-luc, gal4MyoD along with either an empty expression vector or various active signaling molecules as indicated. (E) C2C12 cells were transfected with 3MEF3-luc in combination with vectors encoding signaling molecules as indicated. WCE were made for luciferase activity determination either 24 h (for assays in A, B, D, and E) or 48 h (for assays in C) after cells were grown in DM. All of the above-mentioned experiments were repeated three times, and a representative is shown. The numbers on top of each bar indicate the fold activation.