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. 2002 Jun;13(6):2016–2030. doi: 10.1091/mbc.02-03-0036

Figure 2.

Figure 2

Cellular localization of B23 splicing variants. HeLa cells grown on coverslips were transfected with pEGFPC1-hB23.1 (upper panels) or -hB23.2 (bottom panels). Thirty hours after transfection, cells on coverslips were fixed with 3% paraformaldehyde (first column) or were treated with 0.5% Triton X-10 0 followed by fixation with 3% paraformaldehyde (second column). Triton X-100–treated cells were further digested with DNase I (third column) or RNase A (fourth column) followed by extraction with 0.25 M ammonium sulfate before fixation with paraformaldehyde. DNA stained with Hoechst 33258 (blue) and GFP-tagged proteins (green) were visualized under a fluorescent microscope. The bar at the right bottom indicates 10 μm.