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Primers used for RT-qPCR.
| Genes | Primers (5′– 3′) (F: forward; R: reverse) |
|---|---|
| Bmp2 | F:ACACAGGGACACACCAACCAT |
| R:TGTGACCAGCTGTGTTCATCTTG | |
| Gapdh | F:AGTGCCAGCCTCGTCTCATA |
| R:GGGTTTCCCGTTGATGACCA | |
| Opn | F:CAGTCGATGTCCCTGACGG |
| R:GTTGCTGTCCTGATCAGAGG | |
| Runx2 | F:CACAAGTGCGGTGCAAACTT |
| R:AATGACTCGGTTGGTCTCGG |
Bmp2: bone morphogenetic protein 2. Gapdh: glyceraldehyde-3-phosphate dehydrogenase. Opn: osteopontin. Runx2: runt related transcription factor 2.
