Skip to main content
. 2002 Jun;13(6):2057–2068. doi: 10.1091/mbc.01-12-0571

Figure 6.

Figure 6

W-13 effects on MAPK signaling pathway. Dissection of the MAPK signaling pathway. Serum starved COS-1 cells grown in 100-mm dishes were incubated with W-13 (10 μg/ml) for 60 min, and the last 15 min with the indicated concentrations of EGF, at 37°C. Lysates with equal amount of protein were electrophoresed and the stimulation of EGFR-phosphotyrosine (RC-20) (A), the Ras activation (Ras-GTP) assessed by GST-RBD pull down (B), and the MEK activation (C) were analyzed by Western blotting.