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. 2002 Jun;13(6):2057–2068. doi: 10.1091/mbc.01-12-0571

Figure 8.

Figure 8

Dose-dependent tyrosine phosphorylation of EGFR and MEK activation, using the CaM kinase II inhibitor, KN-93. COS-1 cells were incubated with the indicated concentration of KN-93 or W-13 (10 μg/ml) for 60 min at 37°C (A). An equal amount of protein was electrophoresed and analyzed with antiphosphotyrosine antibody (RC-20) or MEK activation detected by Western blotting with antiphospho-MEK. Also, COS-1 cells were preincubated with10 μg/ml Ab225 for 20 min at 37°C, and the effect of KN-93 (50 μM) is shown (B).